sacB [2019-02-19 20:12:45]

levansucrase

locus: BSU34450

pI: 6.18

mw: 52.80 kDa

protein length: 473 aa

gene length: 1422 bp

function: utilization of sucrose, production of levan

product: levansucrase

essential: no

ec: 2.4.1.10

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,536,012 3,537,433

The protein

Catalyzed reaction/ biological activity

• Sucrose (6)-beta-D-fructofuranosyl-(2)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 68 family (according to Swiss-Prot)

Structure

• [PDB|1PT2] (complex with sucrose), [PDB|1OYG]

[SW|Localization]

• secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]-[gene|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB]
  description: [Pubmed|11739774]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2428811], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|2428811], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY]: antitermination, /antitermination via binding to a [SW|RNA switch], in [regulon|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY regulon]

  regulation

  • the [SW|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• BKE34450 ([gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
• BKK34450 ([gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG

lacZ fusion

• pGP564 (in [protein|search|pAC7]), a series of RAT mutations in [protein|search|pAC7], all available in [SW|Jrg Stlke]'s lab

References

Reviews

• 20735481

Original publications

• 6402497,2428811,18197985,18957862,2105292,11739774,3039303,22247149,26256357,26600431,26646447,28871528,29131629,28433290,29794222,30301900,30703417