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sacB [2019-02-19 20:12:45]
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sacB [2019-02-19 20:12:45]

levansucrase
Locus
BSU34450
Isoelectric point
6.18
Molecular weight
52.80 kDa
Protein length
473 aa Sequence Blast
Gene length
1422 bp Sequence Blast
Function
utilization of sucrose, production of levan
Product
levansucrase
Essential
no
E.C.
2.4.1.10
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,536,012 3,537,433

The protein

Catalyzed reaction/ biological activity

  • Sucrose (6)-beta-D-fructofuranosyl-(2)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family

  • glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Structure

  • 1PT2 (complex with sucrose), 1OYG
  • Localization

  • secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • SacY: antitermination, /antitermination via binding to a RNA switch, in SacY regulon
  • Regulation

  • the RNA switch RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • BKE34450 (sacB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
  • BKK34450 (sacB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
  • LacZ fusion

  • pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Jrg Stlke's lab
  • References

    Reviews

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    Original publications

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